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Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
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December 16, 2011
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A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.
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Skiles, M. L., Sahai, S., Blanchette, J. O. Tracking Hypoxic Signaling within Encapsulated Cell Aggregates. J. Vis. Exp. (58), e3521, doi:10.3791/3521 (2011).
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