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Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

English

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10,436 Views

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09:14 min

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December 16, 2011

DOI:

10.3791/3521-v

DOI:
10.3791/3521-v

•

09:14 min
December 16, 2011

•

2 Views
, ,
1Biomedical Engineering Program,University of South Carolina, 2Chemical Engineering Department,University of South Carolina

Summary

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A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.

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Cite this Article

Skiles, M. L., Sahai, S., Blanchette, J. O. Tracking Hypoxic Signaling within Encapsulated Cell Aggregates. J. Vis. Exp. (58), e3521, doi:10.3791/3521 (2011).

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