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Parallel-Platten-Flow-Kammer und Continuous Flow Circuit endothelialen Vorläuferzellen unter Laminar Flow Schubspannung Bewerten
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Parallel-Platten-Flow-Kammer und Continuous Flow Circuit endothelialen Vorläuferzellen unter Laminar Flow Schubspannung Bewerten

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42,858 Views

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12:43 min

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January 17, 2012

DOI:

10.3791/3349-v

DOI:
10.3791/3349-v

•

12:43 min
January 17, 2012

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20 Views
, , , , , , , , , , ,
1Department of Surgery,Duke University Medical Center, 2Department of Biomedical Engineering,Duke University , 3School of Medicine,University of Pennsylvania , 4Department of Medicine, Division of Cardiology,Duke University Medical Center

Summary

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Wir beschreiben eine Methode, um unter adhärenten Zellen eine laminare Strömung Schubspannung in einem sterilen kontinuierlichen Kreislauf. Die Zellen "Haftung kann Morphologie durch die transparente Kammer untersucht werden, Proben aus der Schaltung für Metabolit-Analyse und-Zellen nach Scher-Belastung für zukünftige Experimente oder Kultur geerntet wurden.

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Lane, W. O., Jantzen, A. E., Carlon, T. A., Jamiolkowski, R. M., Grenet, J. E., Ley, M. M., Haseltine, J. M., Galinat, L. J., Lin, F., Allen, J. D., Truskey, G. A., Achneck, H. E. Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress. J. Vis. Exp. (59), e3349, doi:10.3791/3349 (2012).

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