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Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

DOI:
10.3791/3349-v

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12:43 min

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January 17, 2012

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, , , , , , , , , , ,
1Department of Surgery,Duke University Medical Center, 2Department of Biomedical Engineering,Duke University , 3School of Medicine,University of Pennsylvania , 4Department of Medicine, Division of Cardiology,Duke University Medical Center

Chapitres

  • 00:05Titre
  • 02:20Flow Circuit Assembly
  • 05:30Flow Chamber Assembly
  • 08:17Perfusate-sample Collection
  • 09:08Evaluation of Endothelial Progenitor Cells under Laminar Flow Shear Stress
  • 11:12Conclusion

Summary

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We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.

Tags

Parallel-plate Flow ChamberContinuous Flow CircuitEndothelial Progenitor CellsLaminar Flow Shear StressCell AdhesionCell MorphologyHuman Umbilical Cord Blood-derived Endothelial Progenitor CellsPorcine EPCsSmooth Muscle CellsFibroblastsMicrofluidic ChambersSterilizationCell RecoveryDNA ExtractionRNA ExtractionImmunohistochemistryScanning Electron MicroscopyShear Stress AdjustmentPerfusate Flow RateFluid ViscosityChannel Height And Width

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