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Parallelle plaat flow kamer en Continuous Flow Circuit te endotheliale progenitorcellen Evalueer onder laminaire stroom Afschuifspanning
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Parallelle plaat flow kamer en Continuous Flow Circuit te endotheliale progenitorcellen Evalueer onder laminaire stroom Afschuifspanning

Nederlands

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42,858 Views

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12:43 min

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January 17, 2012

DOI:

10.3791/3349-v

DOI:
10.3791/3349-v

•

12:43 min
January 17, 2012

•

20 Views
, , , , , , , , , , ,
1Department of Surgery,Duke University Medical Center, 2Department of Biomedical Engineering,Duke University , 3School of Medicine,University of Pennsylvania , 4Department of Medicine, Division of Cardiology,Duke University Medical Center

Summary

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We beschrijven een methode om onder hechtende cellen aan laminaire stroming shear stress in een steriele continue stroom circuit. De cellen 'hechting, kan morfologie worden bestudeerd door de transparante kamer, monsters verkregen van het circuit voor de metaboliet-analyse en-cellen geoogst na afschuiving blootstelling voor toekomstige experimenten of cultuur.

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Lane, W. O., Jantzen, A. E., Carlon, T. A., Jamiolkowski, R. M., Grenet, J. E., Ley, M. M., Haseltine, J. M., Galinat, L. J., Lin, F., Allen, J. D., Truskey, G. A., Achneck, H. E. Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress. J. Vis. Exp. (59), e3349, doi:10.3791/3349 (2012).

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