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Colorectal Cancer Cell oppervlakte-eiwit Profiling Met behulp van een antilichaam Microarray en fluorescentie Multiplexing
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Immunology and Infection
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JoVE Journal Immunology and Infection
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Colorectal Cancer Cell oppervlakte-eiwit Profiling Met behulp van een antilichaam Microarray en fluorescentie Multiplexing

Nederlands

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13,853 Views

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15:17 min

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September 25, 2011

DOI:

10.3791/3322-v

DOI:
10.3791/3322-v

•

15:17 min
September 25, 2011

•

6 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Surgery,Royal Prince Alfred Hospital, 3Department of Anatomical Pathology,Department of Anatomical Pathology, 4Department of Medicine,Concord Repatriation General Hospital

Summary

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Beschreven wij een procedure voor het uiteenvallen van colorectale kanker (CRC) levensvatbare enkele cellen, die vervolgens worden opgevangen op maat antilichaam microarrays herkennen van oppervlakte-antigenen (DotScan CRC microarray) te produceren. Subpopulaties van cellen gebonden aan de microarray kan worden geprofileerd door fluorescentie multiplexen behulp van monoklonale antilichamen gelabeld met een fluorescerende kleurstoffen.

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Zhou, J., Belov, L., Solomon, M. J., Chan, C., Clarke, S. J., Christopherson, R. I. Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing. J. Vis. Exp. (55), e3322, doi:10.3791/3322 (2011).

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