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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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免疫学と感染
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JoVE Journal 免疫学と感染
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

13,852 Views

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15:17 min

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September 25, 2011

DOI:

10.3791/3322-v

DOI:
10.3791/3322-v

•

15:17 min
September 25, 2011

•

6 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2外科,Royal Prince Alfred Hospital, 3Department of Anatomical Pathology,Department of Anatomical Pathology, 4医学部,Concord Repatriation General Hospital

概要

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We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.

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Cite this Article

Zhou, J., Belov, L., Solomon, M. J., Chan, C., Clarke, S. J., Christopherson, R. I. Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing. J. Vis. Exp. (55), e3322, doi:10.3791/3322 (2011).

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