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약물 과민성의 IGE - 중재 메커니즘을 조사 Basophil 인증 시험
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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약물 과민성의 IGE - 중재 메커니즘을 조사 Basophil 인증 시험

English

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33,015 Views

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10:22 min

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September 16, 2011

DOI:

10.3791/3263-v

DOI:
10.3791/3263-v

•

10:22 min
September 16, 2011

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12 Views
, , , , , ,
1Department of Molecular Biology,University of Salzburg, 2Department of Neurology,Paracelsus Medical University, 3Department of Dermatology,Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy,University of Salzburg

Summary

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Basophil 인증 테스트 IGE에 의존 알레르기의 탐지를위한 강력한 도구입니다<em> 체외에서</em>. 여기, basophil 활성화 시험에 대한 최적화된 프로토콜이 약물 과민성을 조사하는 데 사용됩니다. 공유 결합 약물 단백질 conjugates 및 물리 특성의 효율적인 생산 방법을 설명합니다.

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Basophil Activation TestInvestigationIgE-Mediated MechanismsDrug HypersensitivityNon-steroidal Anti-inflammatory DrugsPropyphenazoneDiclofenacType I-like Allergic ReactionsClinical PracticePatient HistorySkin TestingOral Provocation TestingIgE-mediated PathomechanismIn Vitro MethodHuman BasophilsDrug-allergic PatientsIgE Receptor CrosslinkingAllergic Effector MoleculesCD63 Surface ExpressionFlow CytometryLow Molecular Weight DrugsConjugatesHuman Serum Albumin (HSA)Carboxyl Group

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Steiner, M., Harrer, A., Lang, R., Schneider, M., Ferreira, F., Hawranek, T., Himly, M. Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity. J. Vis. Exp. (55), e3263, doi:10.3791/3263 (2011).

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