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薬物過敏症におけるIgEが介在する機序の解明のための好塩基球の活性化試験
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Please note that all translations are automatically generated. Click here for the English version.

薬物過敏症におけるIgEが介在する機序の解明のための好塩基球の活性化試験

日本語

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33,096 Views

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10:22 min

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September 16, 2011

DOI:

10.3791/3263-v

DOI:
10.3791/3263-v

•

10:22 min
September 16, 2011

•

12 Views
, , , , , ,
1Department of Molecular Biology,University of Salzburg, 2Department of Neurology,Paracelsus Medical University, 3Department of Dermatology,Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy,University of Salzburg

Summary

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好塩基球の活性化の試験は、IgE依存性のアレルギーを検出するための強力なツールです。<em> in vitroで</em>。ここでは、好塩基球の活性化のテスト用に最適化されたプロトコルは、薬物過敏症を調査するために使用されます。共有結合薬剤 - タンパク質複合体とその物理化学的特性評価の効率的な製造方法について説明する。

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Steiner, M., Harrer, A., Lang, R., Schneider, M., Ferreira, F., Hawranek, T., Himly, M. Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity. J. Vis. Exp. (55), e3263, doi:10.3791/3263 (2011).

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