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Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

33,094 Views

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10:22 min

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September 16, 2011

DOI:

10.3791/3263-v

DOI:
10.3791/3263-v

•

10:22 min
September 16, 2011

•

12 Views
, , , , , ,
1Department of Molecular Biology,University of Salzburg, 2Department of Neurology,Paracelsus Medical University, 3Department of Dermatology,Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy,University of Salzburg

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Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

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Basophil Activation TestInvestigationIgE-Mediated MechanismsDrug HypersensitivityNon-steroidal Anti-inflammatory DrugsPropyphenazoneDiclofenacType I-like Allergic ReactionsClinical PracticePatient HistorySkin TestingOral Provocation TestingIgE-mediated PathomechanismIn Vitro MethodHuman BasophilsDrug-allergic PatientsIgE Receptor CrosslinkingAllergic Effector MoleculesCD63 Surface ExpressionFlow CytometryLow Molecular Weight DrugsConjugatesHuman Serum Albumin (HSA)Carboxyl Group

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Steiner, M., Harrer, A., Lang, R., Schneider, M., Ferreira, F., Hawranek, T., Himly, M. Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity. J. Vis. Exp. (55), e3263, doi:10.3791/3263 (2011).

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