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Preparación de las muestras, imágenes y análisis de protocolos para la microscopía de barrido filo de la navaja
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10:25 min
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December 09, 2011
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1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3Department of Electrical and Computer Engineering,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University
Summary
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El proceso completo, desde la preparación del cerebro muestra de imágenes en serie de seccionamiento con el microscopio de filo de la navaja, a la visualización y análisis de datos se describe. Esta técnica se utiliza actualmente para obtener datos del cerebro del ratón, pero es aplicable a otros órganos, otras especies.
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Cite this Article
Choe, Y., Mayerich, D., Kwon, J., Miller, D. E., Sung, C., Chung, J. R., Huffman, T., Keyser, J., Abbott, L. C. Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy. J. Vis. Exp. (58), e3248, doi:10.3791/3248 (2011).
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