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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
JoVE Journal
生物工学
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JoVE Journal 生物工学
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

DOI:
10.3791/3248-v

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10:25 min

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December 09, 2011

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, , , , , , , ,
1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3電気・コンピュータ工学科,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University

章

  • 00:05標題
  • 01:27Specimen Preparation: Golgi-Cox Method
  • 03:20Specimen Preparation: Nissl Method
  • 04:39Specimen Preparation: India Ink Method
  • 05:37KESM Setup and Imaging
  • 06:38Image Processing, Data Visualization and Analysis
  • 08:12Representative Results of KESM
  • 10:00Conclusion

概要

自動翻訳

自動翻訳

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.

タグ

Specimen PreparationImagingAnalysis ProtocolsKnife-edge Scanning MicroscopyHigh-throughput MicroscopyHigh-resolution Microscopy3D MicroscopyNeuroanatomical DataSubmicrometer ResolutionWhole-brain-scale DataNeuronal NetworksVascular NetworksCell Body DistributionConnectomics ResearchMicrocirculation ResearchHemodynamic ResearchStereology ResearchFixingStainingEmbeddingKESM Configuration And SetupSectioning And Imaging With KESMImage ProcessingData PreparationData VisualizationData Analysis

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