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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

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10:25 min

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December 09, 2011

DOI:

10.3791/3248-v

DOI:
10.3791/3248-v

•

10:25 min
December 09, 2011

•

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1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3Elektrik ve Bilgisayar Mühendisliği Bölümü,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University

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The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.

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Choe, Y., Mayerich, D., Kwon, J., Miller, D. E., Sung, C., Chung, J. R., Huffman, T., Keyser, J., Abbott, L. C. Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy. J. Vis. Exp. (58), e3248, doi:10.3791/3248 (2011).

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