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Chapters
- 00:05Title
- 01:17Isolation of Peripheral Blood Mononuclear Cells (PBMC)
- 03:16Expansion of γδ T Cells
- 05:55Phenotypic Analysis by Flow Cytometry
- 06:57IFN-gamma Production Assay
- 09:40Representative Results
- 12:33Conclusion
Γδ T hücreleri periferik kan mononükleer hücreleri (hücre) genişletmek için bir yöntem açıklanmıştır. Hücre-kökenli γδ T hücreleri uyarılır ve zoledronate ve interlökin-2 (IL-2) ile genişletilir. Γδ T hücrelerinin büyük ölçekli genişlemesi kanser otolog hücresel immünoterapi uygulanabilir.
Tags
Human Peripheral Bloodγδ T CellsExpansionZoledronateStress-induced AntigensAnti-tumor ActivityAnti-infective ActivityVγ9Vδ2 T Cell ReceptorMajor Histocompatibility Complex-independentCytolytic FunctionsTh1-like Effector FunctionsCancer ImmunotherapyPhosphoantigens(E)-4-hydroxy-3-methyl-but-2-enyl Pyrophosphate (HMBPP)Isoprenoid BiosynthesisIsopentenyl Pyrophosphate (IPP)Mevalonate PathwayDysregulationTumor CellsAminobisphosphonatesPamidronateFarnesyl Pyrophosphate Synthase (FPPS)Intracellular Levels Of IPP
