/
/
Expansão do sangue periférico humano γδ células T usando zoledronato
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:17Isolation of Peripheral Blood Mononuclear Cells (PBMC)
- 03:16Expansion of γδ T Cells
- 05:55Phenotypic Analysis by Flow Cytometry
- 06:57IFN-gamma Production Assay
- 09:40Representative Results
- 12:33Conclusion
Um método para expandir células T γδ a partir de células mononucleares do sangue periférico (PBMC) é descrito. PBMC células derivadas de T γδ são estimuladas e expandida usando zoledronato e interleucina-2 (IL-2). Expansão em grande escala de células T γδ podem ser aplicadas a imunoterapia celular autóloga de câncer.
Tags
Human Peripheral Bloodγδ T CellsExpansionZoledronateStress-induced AntigensAnti-tumor ActivityAnti-infective ActivityVγ9Vδ2 T Cell ReceptorMajor Histocompatibility Complex-independentCytolytic FunctionsTh1-like Effector FunctionsCancer ImmunotherapyPhosphoantigens(E)-4-hydroxy-3-methyl-but-2-enyl Pyrophosphate (HMBPP)Isoprenoid BiosynthesisIsopentenyl Pyrophosphate (IPP)Mevalonate PathwayDysregulationTumor CellsAminobisphosphonatesPamidronateFarnesyl Pyrophosphate Synthase (FPPS)Intracellular Levels Of IPP
