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12:45 min
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August 16, 2011
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Summary
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Biz enfekte hücrelerden yüksek saflıkta herpesvirus nucleocapsid DNA izole süreci tanımlamak. Çözüm yakalanan son DNA ideal, yüksek verimlilik sıralama, yüksek sadakat PCR reaksiyonlar, ve yeni virüs rekombinantlar üretmek için transfections için uygun, yüksek konsantrasyon ve saflık.
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Viral DNANucleocapsidsHost Cell ContaminantsDownstream ApplicationsViral Genome SequencingHost DNA RemovalViral Recombinant StrainsPurified PlasmidLinear Viral DNAExtractionsDensity-based CentrifugationPurified Viral CapsidsCellular ProteinsOD260/280 RatioYields Of Viral DNALong-term StorageHigh-throughput SequencingHigh Fidelity PCR ReactionsTransfections
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Cite this Article
Szpara, M. L., Tafuri, Y. R., Enquist, L. W. Preparation of Viral DNA from Nucleocapsids. J. Vis. Exp. (54), e3151, doi:10.3791/3151 (2011).
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