需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Preparation of Viral DNA from Nucleocapsids
20,597 Views
•
12:45 min
•
August 16, 2011
DOI:
Summary
Automatically generated
We describe the process of isolating high purity herpesvirus nucleocapsid DNA from infected cells. The final DNA captured from solution is of high concentration and purity, making it ideally suited for high-throughput sequencing, high fidelity PCR reactions, and transfections to produce new viral recombinants.
Explore More Videos
Viral DNANucleocapsidsHost Cell ContaminantsDownstream ApplicationsViral Genome SequencingHost DNA RemovalViral Recombinant StrainsPurified PlasmidLinear Viral DNAExtractionsDensity-based CentrifugationPurified Viral CapsidsCellular ProteinsOD260/280 RatioYields Of Viral DNALong-term StorageHigh-throughput SequencingHigh Fidelity PCR ReactionsTransfections
Read Article
Cite this Article
Szpara, M. L., Tafuri, Y. R., Enquist, L. W. Preparation of Viral DNA from Nucleocapsids. J. Vis. Exp. (54), e3151, doi:10.3791/3151 (2011).
Copy