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Preparation of Viral DNA from Nucleocapsids
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Preparation of Viral DNA from Nucleocapsids

Preparation of Viral DNA from Nucleocapsids

20,603 Views

12:45 min

August 16, 2011

DOI:

12:45 min
August 16, 2011

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Summary

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We describe the process of isolating high purity herpesvirus nucleocapsid DNA from infected cells. The final DNA captured from solution is of high concentration and purity, making it ideally suited for high-throughput sequencing, high fidelity PCR reactions, and transfections to produce new viral recombinants.

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