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Journal
/
Immunology and Infection
/
活细胞成像
枯草芽孢杆菌
肺炎链球菌
使用自动定时显微镜
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Live Cell Imaging of
Bacillus subtilis
and
Streptococcus pneumoniae
using Automated Time-lapse Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
活细胞成像
枯草芽孢杆菌
肺炎链球菌
使用自动定时显微镜
DOI:
10.3791/3145-v
•
07:31 min
•
July 28, 2011
•
Imke G. de Jong
,
Katrin Beilharz
,
Oscar P. Kuipers
,
Jan- Willem Veening
1
Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology
,
University of Groningen
Chapters
00:05
Title
01:10
Preparation of B. subtilis Cultures
02:18
Preparation of the Microscope Sample
05:14
Time-lapse Fluorescence Microscopy
06:36
Representative B. subtilis Outgrowth Data
07:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了一步一步的程序，以监控不同细菌的单细胞在使用自动荧光时间推移显微镜的行为。此外，我们提供指引如何分析显微镜图像。
Tags
Live Cell Imaging
Bacillus Subtilis
Streptococcus Pneumoniae
Automated Time-lapse Microscopy
Single Cell Studies
Microscopy Method
Fluorescence Microscopy
Growth And Division
Phylogenetic Lineage Trees
Cell History And Ancestry
Gene Expression Dynamics
Protein Localization
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