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Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

41,769 Views

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07:31 min

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July 28, 2011

DOI:

10.3791/3145-v

DOI:
10.3791/3145-v

•

07:31 min
July 28, 2011

•

5 Views
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1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology,University of Groningen

Summary

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This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy. Furthermore, we provide guidelines how to analyze the microscopy images.

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Cite this Article

de Jong, I. G., Beilharz, K., Kuipers, O. P., Veening, J. W. Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy. J. Vis. Exp. (53), e3145, doi:10.3791/3145 (2011).

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