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Misurazione della carica batterica e Risposte immunitarie in topi infettati con Listeria monocytogenes</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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Misurazione della carica batterica e Risposte immunitarie in topi infettati con Listeria monocytogenes</em

italiano

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30,571 Views

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12:50 min

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August 09, 2011

DOI:

10.3791/3076-v

DOI:
10.3791/3076-v

•

12:50 min
August 09, 2011

•

17 Views
, , ,
1St Vincent’s Institute, Department of Medicine,The University of Melbourne, 2Department of Microbiology and Immunology,The University of Melbourne

Summary

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Listeria monocytogenes è un organismo modello per studiare le risposte immunitarie e la suscettibilità genetica a batteri intracellulari nei topi. Questo metodo permette di misurare la carica batterica e di generare monocellulari sospensioni del fegato e della milza di topi per l'analisi FACS per determinare cambiamenti nelle cellule del sistema immunitario dovuta a infezione da Listeria.

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Wang, N., Strugnell, R., Wijburg, O., Brodnicki, T. Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes. J. Vis. Exp. (54), e3076, doi:10.3791/3076 (2011).

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