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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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August 09, 2011
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Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.
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Bacterial LoadImmune ResponsesMiceListeria MonocytogenesGram-positiveIntracellular PathogenSystemic InfectionSpleenLiverCD8α+ Dendritic CellsKupffer CellsPhagocytosedBacterial ProteinsPhagosomeCytosolInnate Immune CellsMonocytesNeutrophilsNK CellsDendritic CellsBactericidal MechanismsCD8+ T CellsClearance Of ListeriaHost Immune ResponsesInbred Mouse StrainsGenetic Studies
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Wang, N., Strugnell, R., Wijburg, O., Brodnicki, T. Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes. J. Vis. Exp. (54), e3076, doi:10.3791/3076 (2011).
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