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Lifetime Imaging di fluorescenza di rotori molecolari nelle cellule viventi
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Lifetime Imaging di fluorescenza di rotori molecolari nelle cellule viventi

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24,377 Views

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09:45 min

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February 09, 2012

DOI:

10.3791/2925-v

DOI:
10.3791/2925-v

•

09:45 min
February 09, 2012

•

4 Views
, , , ,
1Department of Physics,King’s College London, 2Department of Chemistry,Imperial College London , 3PhotoBiotics Ltd

Summary

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Lifetime Imaging di fluorescenza (FLIM) è emersa come una tecnica fondamentale per l'immagine l'ambiente e l'interazione di proteine ​​specifiche e coloranti nelle cellule viventi. FLIM fluorescenti di rotori molecolari consente mappatura di viscosità in cellule viventi.

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Suhling, K., Levitt, J. A., Chung, P. H., Kuimova, M. K., Yahioglu, G. Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells. J. Vis. Exp. (60), e2925, doi:10.3791/2925 (2012).

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