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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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09:45 min

February 09, 2012

DOI:

09:45 min
February 09, 2012

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Summary

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Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.

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