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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

DOI:
10.3791/2925-v

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09:45 min

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February 09, 2012

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, , , ,
1Department of Physics,King’s College London, 2Department of Chemistry,Imperial College London , 3PhotoBiotics Ltd

Chapters

  • 00:05Title
  • 01:43Staining Cells with Molecular Rotors
  • 02:57Fluorescence Lifetime Imaging Microscopy (FLIM) of Fluorescent Molecular Rotors in Cells
  • 04:50FLIM Data Analysis
  • 07:59Representative FLIM Results
  • 09:27Conclusion

Summary

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Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.

Tags

Fluorescence Lifetime ImagingMolecular RotorsLiving CellsDiffusionChemical ReactionsBiological ProcessesIntracellular EventsViscosityDiseaseMalfunctionCellular LevelBulk ViscosityMicroviscosityFluorescence TechniquesOptical TechniquesNon-destructiveFluorescent Molecular RotorsFluorescence LifetimesQuantum YieldsViscosity Maps

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