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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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JoVE Journal Biyomühendislik
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

DOI:
10.3791/2925-v

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09:45 min

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February 09, 2012

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, , , ,
1Fizik Bölümü,King’s College London, 2Kimya Bölümü,Imperial College London , 3PhotoBiotics Ltd

Bölümler

  • 00:05Başlık
  • 01:43Staining Cells with Molecular Rotors
  • 02:57Fluorescence Lifetime Imaging Microscopy (FLIM) of Fluorescent Molecular Rotors in Cells
  • 04:50FLIM Data Analysis
  • 07:59Representative FLIM Results
  • 09:27Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.

Etiketler

Fluorescence Lifetime ImagingMolecular RotorsLiving CellsDiffusionChemical ReactionsBiological ProcessesIntracellular EventsViscosityDiseaseMalfunctionCellular LevelBulk ViscosityMicroviscosityFluorescence TechniquesOptical TechniquesNon-destructiveFluorescent Molecular RotorsFluorescence LifetimesQuantum YieldsViscosity Maps

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