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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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February 09, 2012
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Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.
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Fluorescence Lifetime ImagingMolecular RotorsLiving CellsDiffusionChemical ReactionsBiological ProcessesIntracellular EventsViscosityDiseaseMalfunctionCellular LevelBulk ViscosityMicroviscosityFluorescence TechniquesOptical TechniquesNon-destructiveFluorescent Molecular RotorsFluorescence LifetimesQuantum YieldsViscosity Maps
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Suhling, K., Levitt, J. A., Chung, P. H., Kuimova, M. K., Yahioglu, G. Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells. J. Vis. Exp. (60), e2925, doi:10.3791/2925 (2012).
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