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Bioengineering
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在活细胞中的荧光寿命成像分子转子
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Please note that all translations are automatically generated.
Click here for the English version.
在活细胞中的荧光寿命成像分子转子
DOI:
10.3791/2925-v
•
09:45 min
•
February 09, 2012
•
Klaus Suhling
,
James A. Levitt
,
Pei- Hua Chung
,
Marina. K. Kuimova
,
Gokhan Yahioglu
1
Department of Physics
,
King’s College London
,
2
Department of Chemistry
,
Imperial College London
,
3
PhotoBiotics Ltd
Chapters
00:05
Title
01:43
Staining Cells with Molecular Rotors
02:57
Fluorescence Lifetime Imaging Microscopy (FLIM) of Fluorescent Molecular Rotors in Cells
04:50
FLIM Data Analysis
07:59
Representative FLIM Results
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
荧光寿命成像(FLIM的)已经成为一项关键技术,对图像的环境和特定蛋白质在活细胞染料的相互作用。 FLIM的荧光分子转子允许映射在活细胞中的粘度。
Tags
Fluorescence Lifetime Imaging
Molecular Rotors
Living Cells
Diffusion
Chemical Reactions
Biological Processes
Intracellular Events
Viscosity
Disease
Malfunction
Cellular Level
Bulk Viscosity
Microviscosity
Fluorescence Techniques
Optical Techniques
Non-destructive
Fluorescent Molecular Rotors
Fluorescence Lifetimes
Quantum Yields
Viscosity Maps
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