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Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
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Neuroscience
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JoVE Journal Neuroscience
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

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14,265 Views

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09:53 min

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July 13, 2011

DOI:

10.3791/2841-v

DOI:
10.3791/2841-v

•

09:53 min
July 13, 2011

•

2 Views
, , , , , ,
1College of Veterinary Medicine,Western University of Health Sciences, 2Graduate College of Biomedical Sciences,Western University of Health Sciences, 3ReadiSorb,Products

Summary

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A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.

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Screening AssayOxidative StressFeline Astrocyte Cell LineVirus-induced Neuronal DamageCentral Nervous System (CNS)AstrocytesReactive Oxygen Species (ROS)CM-H2DCFDAFlow CytometryGreen FluorescenceOxidative StatePathogenesis InvestigationOxygen RadicalRegentCellular ConditionsHydrogen Peroxide

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Cite this Article

Testa, M. P., Alvarado, O., Wournell, A., Lee, J., Guilford, F. T., Henriksen, S. H., Phillips, T. R. Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5. J. Vis. Exp. (53), e2841, doi:10.3791/2841 (2011).

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