/
/
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
14,265 Views
•
09:53 min
•
July 13, 2011
DOI:
Summary
Automatically generated
A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.
Explore More Videos
Read Article
Cite this Article
Testa, M. P., Alvarado, O., Wournell, A., Lee, J., Guilford, F. T., Henriksen, S. H., Phillips, T. R. Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5. J. Vis. Exp. (53), e2841, doi:10.3791/2841 (2011).
Copy