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Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
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Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
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July 13, 2011
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A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.
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Testa, M. P., Alvarado, O., Wournell, A., Lee, J., Guilford, F. T., Henriksen, S. H., Phillips, T. R. Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5. J. Vis. Exp. (53), e2841, doi:10.3791/2841 (2011).
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