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1Department of Medicine,Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program,Memorial Sloan Kettering Cancer Center, 4Howard Hughes,Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics,The Rockefeller University
Chapters
- 00:05Title
- 01:35Dissection of the Mouse Gastrointestinal Tract
- 02:56Whole Mount Sample Preparation and Immunostaining
- 05:10Cryosection Preparation and Immunostaining
- 07:44Representative Imaging Results
- 09:23Conclusion
الخلايا من خلالي كاجال (ICC) هي خلايا تنظيم ضربات القلب من الجهاز (GI) الهضمي. انهم يشكلون شبكات معقدة بين خلايا العضلات الملساء وبعد العقدية الألياف العصبية لتنظيم انقباض الجهاز الهضمي. هنا ، نقدم أساليب المناعي مستعرضة وكله يشن التصور شبكات المحكمة الجنائية الدولية الفئران.
Tags
VisualizationInterstitial Cells Of Cajal (ICC)MiceGastrointestinal (GI) TractPacemaker CellsSlow WavesPeristalsisEnteric Nervous SystemMotility DisordersKIT Receptor Tyrosine KinaseKIT ImmunostainingICC NetworkKIT ActivityNeoplastic TransformationGastrointestinal Stromal Tumor (GIST)ETV1 Survival FactorICC/GIST LineageTumorigenesisActivating KIT MutationsVisualization MethodsPreviously Published ProtocolsAnoctamin 1 (ANO1)Membrane MarkerImmunofluorescence
