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September 07, 2011
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Un protocollo generale per l'uso di calorimetria isotermica di titolazione per monitorare la termodinamica vincolante per i sistemi biologici con moderata affinità di legame è presentato.
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Isothermal Titration CalorimetryITCMacromolecule-ligand AffinityBinding ReactionThermodynamic PictureBiological SciencesMacromolecular InteractionsExperimental ConditionsBufferTemperatureLigand ConcentrationMacromolecule ConcentrationAccurate DeterminationSample PreparationImpuritiesITC Experiment ControlsStoichiometryAffinityEnthalpyAdditional ExperimentsBuffer ConditionsTemperature ConditionsProtocol
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Duff, Jr., M. R., Grubbs, J., Howell, E. E. Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity. J. Vis. Exp. (55), e2796, doi:10.3791/2796 (2011).
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