ジャーナル
/
生物学
/
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

DOI:
10.3791/2796-v

•

08:45 min

•

September 07, 2011

•

, ,
1Department of Biochemistry, Cellular and Molecular Biology,University of Tennessee

章

  • 00:05標題
  • 01:06Isothermal Titration Calorimetry (ITC) Sample Preparation
  • 02:34The ITC Experiment
  • 05:46ITC Data Analysis
  • 07:20Representative ITC Results
  • 08:21Conclusion

概要

自動翻訳

自動翻訳

A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.

タグ

Isothermal Titration CalorimetryITCMacromolecule-ligand AffinityBinding ReactionThermodynamic PictureBiological SciencesMacromolecular InteractionsExperimental ConditionsBufferTemperatureLigand ConcentrationMacromolecule ConcentrationAccurate DeterminationSample PreparationImpuritiesITC Experiment ControlsStoichiometryAffinityEnthalpyAdditional ExperimentsBuffer ConditionsTemperature ConditionsProtocol

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Titration of Human Coronaviruses Using an Immunoperoxidase Assay

Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms

High-throughput Purification of Affinity-tagged Recombinant Proteins

Biochemical Titration of Glycogen In vitro

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

A Customizable Chamber for Measuring Cell Migration

Read Article