A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.
Duff, Jr., M. R., Grubbs, J., Howell, E. E. Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity. J. Vis. Exp. (55), e2796, doi:10.3791/2796 (2011).