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Isolation of Brain-infiltrating Leukocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Brain-infiltrating Leukocytes

Isolation of Brain-infiltrating Leukocytes

DOI:
10.3791/2747-v

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06:44 min

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June 13, 2011

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,
1Department of Neurology,Mayo Clinic College of Medicine

Chapters

  • 00:05Title
  • 01:04Intracranial Virus Injection
  • 02:15Brain-infiltrating Leukocyte Cell Preparation
  • 04:41Flow Cytometric Immunophenotyping
  • 05:44Representative Immunophenotype of Brain-infiltrating Leukocytes at 24 hr Post-infection
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.

Tags

Brain-infiltrating LeukocytesBILsMiceTheiler’s Murine Encephalomyelitis VirusTMEVIntracranial Injection TechniqueLeukocyte PurificationBrain HomogenizationPercoll GradientCell SuspensionFicoll-Paque Plus GradientImmunophenotypingFlow CytometryInnate Immune CellsGr1+CD11b+F4/80+ CellsImmune ResponseAcute Infection

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