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06:44 min
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June 13, 2011
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A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
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Cite this Article
LaFrance-Corey, R. G., Howe, C. L. Isolation of Brain-infiltrating Leukocytes. J. Vis. Exp. (52), e2747, doi:10.3791/2747 (2011).
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