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Biyomoleküler Algılama istihdam İnterferometrik Yansıtma Görüntüleme Sensörü (IRIS)
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
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Biyomoleküler Algılama istihdam İnterferometrik Yansıtma Görüntüleme Sensörü (IRIS)

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14,362 Views

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11:04 min

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May 03, 2011

DOI:

10.3791/2694-v

DOI:
10.3791/2694-v

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11:04 min
May 03, 2011

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1 Views
, , , , , , , , , , , , ,
1Department of Electrical and Computer Engineering,Boston University , 2Department of Biomedical Engineering,Boston University , 3Center for Advanced Genomics Technology,Boston University , 4Department of Medicine, Section of Infectious Diseases,Boston University School of Medicine, 5Department of Microbiology,Boston University School of Medicine, 6CNR (National Research Council),Istituto di Chimica del Riconoscimento Molecolare

Summary

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SiO Kantitatif, yüksek verimli, gerçek zamanlı ve etiket ücretsiz biyomoleküler algılama (DNA, protein, vb.)<sub> 2</sub> Yüzeyler LED aydınlatma dayanan basit bir interferometrik tekniği, minimal optik bileşenleri ve bir kamera kullanılarak elde edilebilir. İnterferometrik Yansıtma Görüntüleme Sensörü (IRIS) formatlarını mikroarray ucuz, kullanımı kolay, ve uysal.

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Biomolecular DetectionInterferometric Reflectance Imaging Sensor (IRIS)Biomolecular InteractionsDiagnostic ApplicationsProteomic BiomarkersGenomic BiomarkersMolecular AffinitiesMolecular SpecificitiesLabelsImmunoassaysEnzyme-linked Immunosorbent Assay (ELISA)Polymerase Chain Reaction (PCR)Gel Electrophoresis AssaysMass Spectrometry (MS)Label-free Methods

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Lopez, C. A., Daaboul, G. G., Ahn, S., Reddington, A. P., Monroe, M. R., Zhang, X., Irani, R. J., Yu, C., Genco, C. A., Cretich, M., Chiari, M., Goldberg, B. B., Connor, J. H., Ünlü, M. S. Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS). J. Vis. Exp. (51), e2694, doi:10.3791/2694 (2011).

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