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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
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Bioingeniería
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JoVE Revista Bioingeniería
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

14,356 Views

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11:04 min

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May 03, 2011

DOI:

10.3791/2694-v

DOI:
10.3791/2694-v

•

11:04 min
May 03, 2011

•

1 Views
, , , , , , , , , , , , ,
1Department of Electrical and Computer Engineering,Boston University , 2Department of Biomedical Engineering,Boston University , 3Center for Advanced Genomics Technology,Boston University , 4Department of Medicine, Section of Infectious Diseases,Boston University School of Medicine, 5Department of Microbiology,Boston University School of Medicine, 6CNR (National Research Council),Istituto di Chimica del Riconoscimento Molecolare

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Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.

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Biomolecular DetectionInterferometric Reflectance Imaging Sensor (IRIS)Biomolecular InteractionsDiagnostic ApplicationsProteomic BiomarkersGenomic BiomarkersMolecular AffinitiesMolecular SpecificitiesLabelsImmunoassaysEnzyme-linked Immunosorbent Assay (ELISA)Polymerase Chain Reaction (PCR)Gel Electrophoresis AssaysMass Spectrometry (MS)Label-free Methods

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Lopez, C. A., Daaboul, G. G., Ahn, S., Reddington, A. P., Monroe, M. R., Zhang, X., Irani, R. J., Yu, C., Genco, C. A., Cretich, M., Chiari, M., Goldberg, B. B., Connor, J. H., Ünlü, M. S. Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS). J. Vis. Exp. (51), e2694, doi:10.3791/2694 (2011).

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