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Dissection und 2-Photonen-Imaging der peripheren Lymphknoten in Mäusen
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Biology
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JoVE Journal Biology
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Dissection und 2-Photonen-Imaging der peripheren Lymphknoten in Mäusen

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45,629 Views

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16:48 min

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August 23, 2007

DOI:

10.3791/265-v

DOI:
10.3791/265-v

•

16:48 min
August 23, 2007

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20 Views
, ,
1Department of Physiology and Biophysics,University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Summary

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Zwei-Photonen-Imaging hat Lymphozyten Motilität und zellulären Interaktionen im Lymphknoten unter basalen Bedingungen und während einer Immunantwort Antwort 1 aufgedeckt. Hier zeigen wir, adoptiven Transfer von T-Zellen, Isolierung von Lymphknoten und Imaging-Motilität der CD4 + T-Zellen in der explantierten Lymphknoten.

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Cite this Article

Matheu, M. P., Parker, I., Cahalan, M. D. Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice. J. Vis. Exp. (7), e265, doi:10.3791/265 (2007).

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