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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Biología
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JoVE Revista Biología
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

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16:48 min

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August 23, 2007

DOI:

10.3791/265-v

DOI:
10.3791/265-v

•

16:48 min
August 23, 2007

•

20 Views
, ,
1Department of Physiology and Biophysics,University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Summary

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Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node.

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Dissection2-Photon ImagingPeripheral Lymph NodesMiceAdoptive TransferLabeled T CellsIsolationImaging MotilityCD4+ T CellsExplanted Lymph NodeTwo-photon Imaging Of Immune CellsFluorescently Labeled CellsCell Tracker DyeFluorescent ProteinTail Vein InjectionLymphoid OrgansIsolation Of Lymph NodeExcised Lymph NodeOxygenation Of Perfused MediaTemperatureLaser Power3D Video ImagesNaive CD4+ T Cells

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Cite this Article

Matheu, M. P., Parker, I., Cahalan, M. D. Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice. J. Vis. Exp. (7), e265, doi:10.3791/265 (2007).

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