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Dissection en 2-Photon Imaging van perifere lymfeklieren in Muizen
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Biology
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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Dissection en 2-Photon Imaging van perifere lymfeklieren in Muizen

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45,629 Views

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16:48 min

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August 23, 2007

DOI:

10.3791/265-v

DOI:
10.3791/265-v

•

16:48 min
August 23, 2007

•

20 Views
, ,
1Department of Physiology and Biophysics,University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Summary

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Twee-foton beeldvorming heeft blootgelegd lymfocyten beweeglijkheid en cellulaire interacties binnen de lymfeklier onder basale omstandigheden en gedurende een immuunrespons 1. Hier laten we zien adoptieve overdracht van T-cellen, isolatie van de lymfeklieren, en beeldvorming beweeglijkheid van de CD4 + T-cellen in de geëxplanteerd lymfeklier.

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Cite this Article

Matheu, M. P., Parker, I., Cahalan, M. D. Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice. J. Vis. Exp. (7), e265, doi:10.3791/265 (2007).

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