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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
Capitoli
- 00:05Titolo
- 01:45Mapping Transgenic Flippase CNS Expression Patterns in Larva
- 03:37Mapping Transgenic Flippase CNS Expression Patterns in Adults
- 05:57Determining the Cell Types in which a Transgenic Flippase Expresses
- 06:57Utilizing FLP Expression in Photoreceptors from ET-FLP-x2 Lines
- 08:12Conclusion
We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.
