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iCLIP - 全转录组,蛋白质与RNA相互作用的映射与个别核苷酸分辨率
JoVE Journal
Biology
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JoVE Journal
Biology
iCLIP – Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Please note that all translations are automatically generated.
Click here for the English version.
iCLIP - 全转录组,蛋白质与RNA相互作用的映射与个别核苷酸分辨率
DOI:
10.3791/2638-v
•
10:45 min
•
April 30, 2011
•
Julian Konig
,
Kathi Zarnack
,
Gregor Rot
,
Tomaz Curk
,
Melis Kayikci
,
Blaz Zupan
,
Daniel J. Turner
,
Nicholas M. Luscombe
,
Jernej Ule
1
Laboratory of Molecular Biology
,
Medical Research Council – MRC
,
2
European Bioinformatics Institute
,
EMBL Heidelberg
,
3
Computer and Information Science
,
University of Ljubljana
,
4
Wellcome Trust Genome Campus
,
Wellcome Trust Sanger Institute
Chapters
00:05
Title
01:19
UV Cross-linking of Tissue Culture Cells
02:10
Bead Preparation
02:53
Cell Lysis and Partial RNA Digestion
03:47
Immunoprecipitation
04:26
RNA 5′ End Labelling
05:08
SDS-PAGE and Membrane Transfer
06:27
Gel Purification of cDNA
08:44
Representative Results
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
成绩单的RNA结合蛋白的空间安排,是一种转录后调控的关键因素。因此,我们制定个人核苷酸分辨率的紫外光交联和免疫(iCLIP),允许精确的全基因组RNA结合蛋白的结合位点的映射。
Tags
ICLIP
Transcriptome-wide Mapping
Protein-RNA Interactions
Individual Nucleotide Resolution
RNA-binding Proteins
Post-transcriptional Regulation
Positional Information
Binding Sites
Biochemical Methods
Native Cellular Context
Affinity Purification
Immunoprecipitation
Differential Display
Microarray Analysis
RIP-CHIP
Indirect Interactions
Non-physiological Interactions
CLIP (UV Cross-linking And Immunoprecipitation)
UV Cross-linking
Rigorous Purification Schemes
Denaturing Polyacrylamide Gel Electrophoresis
High-throughput Sequencing Technologies
HITS-CLIP
CLIP-seq
PAR-CLIP
Photoreactive Ribonucleoside Analogs
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