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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
 

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Article DOI: 10.3791/2638-v • 10:45 min • April 30th, 2011
April 30th, 2011 •
1, 2, 3, 3, 1, 3, 4, 2, 1
1Laboratory of Molecular Biology, Medical Research Council - MRC, 2European Bioinformatics Institute, EMBL Heidelberg, 3Computer and Information Science, University of Ljubljana, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute

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The spatial arrangement of RNA-binding proteins on a transcript is a key determinant of post-transcriptional regulation. Therefore, we developed individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) that allows precise genome-wide mapping of the binding sites of an RNA-binding protein.

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ICLIP Transcriptome-wide Mapping Protein-RNA Interactions Individual Nucleotide Resolution RNA-binding Proteins Post-transcriptional Regulation Positional Information Binding Sites Biochemical Methods Native Cellular Context Affinity Purification Immunoprecipitation Differential Display Microarray Analysis RIP-CHIP Indirect Interactions Non-physiological Interactions CLIP (UV Cross-linking And Immunoprecipitation) UV Cross-linking Rigorous Purification Schemes Denaturing Polyacrylamide Gel Electrophoresis High-throughput Sequencing Technologies HITS-CLIP CLIP-seq PAR-CLIP Photoreactive Ribonucleoside Analogs
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