This content is Free Access.
iCLIP – Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
57,669 Views
•
10:45 min
•
April 30, 2011
DOI:
Tags
ICLIPTranscriptome-wide MappingProtein-RNA InteractionsIndividual Nucleotide ResolutionRNA-binding ProteinsPost-transcriptional RegulationPositional InformationBinding SitesBiochemical MethodsNative Cellular ContextAffinity PurificationImmunoprecipitationDifferential DisplayMicroarray AnalysisRIP-CHIPIndirect InteractionsNon-physiological InteractionsCLIP (UV Cross-linking And Immunoprecipitation)UV Cross-linkingRigorous Purification SchemesDenaturing Polyacrylamide Gel ElectrophoresisHigh-throughput Sequencing TechnologiesHITS-CLIPCLIP-seqPAR-CLIPPhotoreactive Ribonucleoside Analogs
Read Article
Cite this Article
Konig, J., Zarnack, K., Rot, G., Curk, T., Kayikci, M., Zupan, B., Turner, D. J., Luscombe, N. M., Ule, J. iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution. J. Vis. Exp. (50), e2638, doi:10.3791/2638 (2011).
Copy