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Chapters
- 00:05Title
- 01:02Introduction
- 01:30Preparations for Slicing
- 03:14Slicing the Brain and Preparation for Culture
- 06:01Transfecting the Slices Using a Gene Gun
- 07:31Recording from Pyramidal Cells in Slices
- 09:10Results: Recordings from Neocortical Pyramidal Cells in Organotypic Slice Culture
- 10:02Conclusion
Tags
Whole Cell RecordingOrganotypic Slice PreparationNeocortexVoltage-gated Potassium ChannelsPyramidal NeuronsGenetic ApproachOrganotypic Culture PreparationLaminar Pattern Of CortexAcute Neocortical SlicesVisually-identified Pyramidal NeuronsInfrared IlluminationDifferential Interference Contrast MicroscopyWhole Cell Patch ClampCurrent-clampVoltage-clampBiolistic TransfectionWild Type Potassium Channel DNAMutant Potassium Channel DNAFunction StudyEpifluorescence MicroscopyGreen Fluorescent Protein (GFP)Adjacent Untransfected Neurons
