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Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
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Neurociência
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JoVE Journal Neurociência
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

DOI:
10.3791/2600-v

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10:29 min

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June 03, 2011

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, , ,
1Department of Anatomy and Neurobiology,University of Tennessee Health Science Center

Capítulos

  • 00:05Título
  • 01:02Introduction
  • 01:30Preparations for Slicing
  • 03:14Slicing the Brain and Preparation for Culture
  • 06:01Transfecting the Slices Using a Gene Gun
  • 07:31Recording from Pyramidal Cells in Slices
  • 09:10Results: Recordings from Neocortical Pyramidal Cells in Organotypic Slice Culture
  • 10:02Conclusion

Summary

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This is a protocol to prepare and maintain a neocortical slice preparation in organotypic culture for the purpose of making electrical recordings from pyramidal neurons.

Tags

Whole Cell RecordingOrganotypic Slice PreparationNeocortexVoltage-gated Potassium ChannelsPyramidal NeuronsGenetic ApproachOrganotypic Culture PreparationLaminar Pattern Of CortexAcute Neocortical SlicesVisually-identified Pyramidal NeuronsInfrared IlluminationDifferential Interference Contrast MicroscopyWhole Cell Patch ClampCurrent-clampVoltage-clampBiolistic TransfectionWild Type Potassium Channel DNAMutant Potassium Channel DNAFunction StudyEpifluorescence MicroscopyGreen Fluorescent Protein (GFP)Adjacent Untransfected Neurons

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