需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
14,042 Views
•
10:29 min
•
June 03, 2011
DOI:
Summary
Automatically generated
This is a protocol to prepare and maintain a neocortical slice preparation in organotypic culture for the purpose of making electrical recordings from pyramidal neurons.
Explore More Videos
Whole Cell RecordingOrganotypic Slice PreparationNeocortexVoltage-gated Potassium ChannelsPyramidal NeuronsGenetic ApproachOrganotypic Culture PreparationLaminar Pattern Of CortexAcute Neocortical SlicesVisually-identified Pyramidal NeuronsInfrared IlluminationDifferential Interference Contrast MicroscopyWhole Cell Patch ClampCurrent-clampVoltage-clampBiolistic TransfectionWild Type Potassium Channel DNAMutant Potassium Channel DNAFunction StudyEpifluorescence MicroscopyGreen Fluorescent Protein (GFP)Adjacent Untransfected Neurons
Read Article
Cite this Article
Foehring, R. C., Guan, D., Toleman, T., Cantrell, A. R. Whole Cell Recording from an Organotypic Slice Preparation of Neocortex. J. Vis. Exp. (52), e2600, doi:10.3791/2600 (2011).
Copy