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Alta MicroRNA profiling Throughput: Multiplex Ottimizzato qRT-PCR a scala nanoliter sul Fluidigm dinamica IFCS ArrayTM
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August 03, 2011
DOI:
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California San Francisco , 2Center for Reproductive Sciences,University of California San Francisco , 3Department of Urology,University of California San Francisco , 4Department of Cell and Tissue Biology,University of California San Francisco , 5Fluidigm Corporation,Fluidigm Corporation , 6Department of Obstetrics and Gynecology,Hadassah-Hebrew University Medical Center, 7UCSF – Helen Diller Family Comprehensive Cancer Center,University of California San Francisco
Summary
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Qui descrivere un ottimizzato trascrittasi inversa multiplex PCR quantitativa (QRT-PCR) protocollo in combinazione con una piattaforma microfluidica come un efficace costi e tempi high-throughput screening per microRNA (miRNA) livelli di espressione, soprattutto quando si lavora con quantità limitate di campione.
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Cite this Article
Moltzahn, F., Hunkapiller, N., Mir, A. A., Imbar, T., Blelloch, R. High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs. J. Vis. Exp. (54), e2552, doi:10.3791/2552 (2011).
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