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December 04, 2010
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Hier beschrijven we een test dat de kracht van micro-injectie in combinatie met fluorescerende werken<em> In situ</em> Hybridisatie om nauwkeurig de nucleaire export kinetiek van mRNA te meten in somatische cellen van zoogdieren.
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MRNA Nuclear Export KineticsMammalian CellsMicroinjectionEukaryotesCytoplasmTranslation MachineryXenopus OocytesYeastDrosophila Derived S2 Cell LineKinetic DataFluorescent In Situ Hybridization (FISH)Splicing Dependent MannerSignal Sequence Coding Region (SSCR)In Vitro Synthesized MRNAPlasmid DNASub-cellular Localization
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Gueroussov, S., Tarnawsky, S. P., Cui, X. A., Mahadevan, K., Palazzo, A. F. Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection. J. Vis. Exp. (46), e2387, doi:10.3791/2387 (2010).
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