Journal
/
生物学
/
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

DOI:
10.3791/2387-v

•

11:32 min

•

December 04, 2010

•

, , , ,
1Department of Biochemistry,University of Toronto

Chapters

  • 00:05Title
  • 01:23Preparation for Microinjection
  • 02:41Intranuclear Microinjection
  • 05:46Cell Fixation and Staining
  • 08:48Representative Results for mRNA Nuclear Export Kinetics
  • 10:23Conclusion

Summary

自动翻译

自动翻译

Here we describe an assay that employs the power of microinjection coupled with fluorescent in situ hybridization in order to accurately measure the nuclear export kinetics of mRNA in mammalian somatic cells.

Tags

MRNA Nuclear Export KineticsMammalian CellsMicroinjectionEukaryotesCytoplasmTranslation MachineryXenopus OocytesYeastDrosophila Derived S2 Cell LineKinetic DataFluorescent In Situ Hybridization (FISH)Splicing Dependent MannerSignal Sequence Coding Region (SSCR)In Vitro Synthesized MRNAPlasmid DNASub-cellular Localization

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Measuring the Kinetics of mRNA Transcription in Single Living Cells

Analysis of Cell Cycle Position in Mammalian Cells

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Read Article