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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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December 04, 2010
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Here we describe an assay that employs the power of microinjection coupled with fluorescent in situ hybridization in order to accurately measure the nuclear export kinetics of mRNA in mammalian somatic cells.
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MRNA Nuclear Export KineticsMammalian CellsMicroinjectionEukaryotesCytoplasmTranslation MachineryXenopus OocytesYeastDrosophila Derived S2 Cell LineKinetic DataFluorescent In Situ Hybridization (FISH)Splicing Dependent MannerSignal Sequence Coding Region (SSCR)In Vitro Synthesized MRNAPlasmid DNASub-cellular Localization
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Gueroussov, S., Tarnawsky, S. P., Cui, X. A., Mahadevan, K., Palazzo, A. F. Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection. J. Vis. Exp. (46), e2387, doi:10.3791/2387 (2010).
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